The in
vitro morphogenetic capacity of olive embryo explants at
different developmental stages, as affected by L-Glutamine, L-Arginine
and 2,4-D |
T. S. PRITSA
and D. G. VOYIATZIS |
Abstract
The role of exogenously applied L-glutamine (Gln) and L-arginine (Arg)
in growth (dry weight), cell division (callus induction) and
differentiation (spherical structure formation), of cotyledonary
explants of olive embryos, cv. Chondrolia Chalkidikis, at three
developmental stages, and of fully developed embryos, was investigated,
in relation to the presence of auxin in the nutrient medium. The
percentage of explants forming spherical structures increased with the
advancement of the embryo development and was particularly high in the
absence of auxin. Histological examination showed these structures to be
either somatic embryos at an early stage of development or organ
initials. The presence of Arg generally inhibited growth and
morphogenetic capacity of explants. Glutamine up to 500 mg.L-1
did not affect growth but in most cases improved slightly callus
induction. There was a significant, negative correlation between the
cumulative concentration of the two amino acids and the parameters
measured.
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