Anionic
peroxidase isoform profiles from calli and barks of pear
cultivars and of the quince rootstock EM A |
DEMETRIOS
PETKOU1, GRIGORIOS DIAMANTIDIS2 and
MILTIADIS VASSILAKAKIS1
1 Laboratory of Pomology, and 2 Laboratory of Agricultural
Chemistry,
School of Agriculture, Aristotle University, 54124 Thessaloniki,
Greece
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Abstract
The anionic peroxidases (E.C. 1.11.1.7.) of three pear (Pyrus
communis L.) cultivars, representing an increasing scale of graft
incompatibility with quince (Cydonia oblonga Mill.), and of the
quince rootstock EM A, were analyzed by discontinuous non-denaturing
polyacrylamide gel electrophoresis. Clear qualitative variations were
detected among the electrophoretic patterns of the pear cultivars. These
variations were observed at peroxidase isoforms that were slow migrating
in the gels and were predominant in the callus extracts instead of the
bark extracts. The roles of the electrophoretic methods and of the
anionic peroxidase isoforms in the prediction of graft incompatibility
are discussed.
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