Abstract A micropropagation technique is described for the multiplication of Bambusa wamin through shoot proliferation. Nodal explants containing axillary buds and obtained from a 10-12 year-old field grown clump of B. wamin were used to initiate cultures. Multiple shoots were induced on Murashige and Skoog’s (MS) medium supplemented with 5.0 mg l-1 6-benzylamino purine (BAP). Rapid shoot multiplication was obtained on alternate subcultures on a BAP and kinetin (Kn) supplemented liquid and a semisolid MS medium for initial few subculture cycles. Later, an average multiplication rate of 4.20-fold was achieved at a 4-week subculture cycle on MS medium supplemented with 2.0 mg l-1 BAP and 0.8 mg l-1 Kn under semisolid conditions alone. In vitro multiplied shoots rooted well on a modified MS medium containing 7.5 mg l-1 1H-indole-3-butyric acid (IBA). The in vitro regenerated plantlets, after hardening and acclimatization, showed 80 - 90% survival when transferred to the field. Abbreviations: MS: Murashige and Skoog (1962) medium; BAP: 6-benzylamino purine; Kn: kinetin; NAA: 1-naphthaleneacetic acid; IBA: 1H-indole-3-butyric acid.
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