In vitro seed germination and plantlet regeneration of  Coelogyne mossiae Rolfe JOSEPH SEBASTINRAJ1, JOHN S. BRITTO1*, PHILIP J. ROBINSON1, VINOTH D. KUMAR1 and SENTHIL S. KUMAR2 1 The Rapinat Herbarium and Center for Molecular Systematics 2 Department of Plant Biology and Plant Biotechnology, St. Joseph’s College (Autonomous), Tiruchirappalli 620 002, India Abstract The effects of different nutrient solutions, organic supplements and plant growth regulators on in vitro seed germination and plantlet development of Coelogyne mossiae were studied. Seed germination was higher in C Knudson (1946) medium (95%) and lower in half-strength Murashige and Skoog (1962) medium (65%). After 5 months, when plantlets had produced 2-3 leaves, they were transferred to hormone-supplemented half-strength Murashige and Skoog medium and Knudson C medium. Half-strength Murashige and Skoog medium supplemented with 0.2 mgl-1 6-benzylamino purine proved better for multiplication of protocorm and healthy shoot induction. After 12 months, to derive seedlings with a height of 7 cm, plantlets were transferred to auxin-supplemented half-strength Murashige and Skoog medium and basic half-strength Murashige and Skoog medium for root induction. High frequency of roots was observed in the medium fortified with 0.1 mgl-1 auxin. Then, rooted plantlets were transferred to a decomposed paper disposal cup containing garden soil, vermicompost, and brickgravals (1:1:1) used for successful hardening.   < back .