- SHORT COMMUNICATION -
Chick yolk sac membrane assay:a novel angiogenesis model |
HONG-MEI WANG1, CHENG-YU LU1, XIU-HONG WANG1,
YONG-LI BAO1, XIANG-YING MENG2, YIN WU1 and YU-XIN LI2
1. Institute of Genetics and Cytology, Northeast Normal University, Changchum 130024, P. R. China
2. Research Center of Agriculture and Medicine Gene Engineering of Ministry of Education, Renmin street 5268, Changchun 130024, P. R. China
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Abstract
Many angiogenesis models have been developed so far, however most of
them have a number of limitations such as requiring skilled operation
and quite long testing times. Based on the conventional chick
chorioallantoic membrane (CAM) angiogenesis assay, we developed a chick
yolk sac membrane (YSM) model, which provides a simple and easy method
in vivo for screening of angiogenesis-affecting drugs. Fertilized chick
eggs (60 hours of incubation) were opened, and their contents were
poured into beakers for 12 hour-incubation and then put the test
solution into silicon rings laid on YSM for another 12 hours of
incubation. The influences of the tested drugs on YSM angiogenesis were
observed dynamically. The results showed that the YSM vascular growth
was obviously inhibited by an angiogenesis inhibitor, the recombinant
human angiostatin (rh-AS). Specially designed software makes YSM assay
simple, timesaving and accurate.
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